Testing photoheterotrophic, autotrophic and fermentation of R.rubrum?

[prime-factor]

I need to test photoheteretrophic, autotrophic and fermentation growth of R.rubrum bacteria, all of which require anaerobic conditions. For fermentation, i am placing the bacteria with fructose in the dark, and for phototrophic in the light with fructose, and for autotrophic in the light with nothing. How should I do this to get some quantitative data??

How do I get anaerobic conditions,and how should i test this?, please help :confused:

[Ouabache]

How should I do this to get some quantitative data?
Have you considered serial dilution and spread plating (http://www2.hendrix.edu/biology/CellWeb/Techniques/microspread.html?)?
How do I get anaerobic conditions?
Have you considered GasPak (http://ftp.ccccd.edu/dcain/CCCCD%20Micro/gaspakjar.htm) culture?

[prime-factor]

Thanks. Unfortunately our school has no equipment such as the GasPak you mentioned :frown:
However :smile:, the bacteria were put in test-tubes filled with sterile water, then covered with oil. Hopefully things will work out.