Exome Sequencing: Chip vs. Sequencing

Thread starter Soaring Crane
Start date Jun 28, 2013

In summary, chip-based exome sequencing uses a microarray or chip to sequence only the exons of the genome, while sequencing-based exome sequencing directly sequences the entire exome. Sequencing-based exome sequencing is generally considered more accurate and can detect rare variants, but both methods have their own strengths and limitations. Chip-based exome sequencing is faster and less expensive, and allows for targeted sequencing of specific regions. However, sequencing-based exome sequencing may have lower coverage and require more data analysis. Currently, both methods are used in clinical settings, but sequencing-based exome sequencing is becoming more popular due to its higher accuracy and ability to detect rare variants.

Jun 28, 2013

Soaring Crane

Is there a difference between an exome chip and exome sequencing?

Thank you.

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Jun 28, 2013

Ygggdrasil

Science Advisor

Insights Author

Gold Member

By exome chip do you mean something like an exome genotyping array? In what context was the term used?

FAQ: Exome Sequencing: Chip vs. Sequencing

What is the difference between chip-based and sequencing-based exome sequencing?

Chip-based exome sequencing involves using a microarray or chip that contains specific DNA probes to capture and sequence only the exons (coding regions) of the genome. Sequencing-based exome sequencing, on the other hand, uses next-generation sequencing technology to directly sequence the entire exome without the need for a microarray or chip.

Which method is more accurate for exome sequencing?

Sequencing-based exome sequencing is generally considered more accurate because it has a higher coverage and can detect rare variants that may be missed by chip-based exome sequencing. However, both methods have their own strengths and limitations.

What are the advantages of chip-based exome sequencing?

Chip-based exome sequencing is generally faster and less expensive compared to sequencing-based exome sequencing. It also allows for targeted sequencing of specific regions of interest, making it useful for targeted gene panels.

What are the limitations of sequencing-based exome sequencing?

Sequencing-based exome sequencing may have lower coverage and may not be able to detect larger structural variations or repeat expansions. It also requires more data analysis and interpretation compared to chip-based exome sequencing.

Which method is more commonly used for clinical exome sequencing?

Currently, both chip-based and sequencing-based exome sequencing are used in clinical settings. However, sequencing-based exome sequencing is becoming more popular due to its higher accuracy and ability to detect rare variants, making it a more comprehensive option for clinical diagnosis.