hello, I'm form Germany and study biotechnology in my third semester and have to find an unknown substance by using an NMR spectrum. Unfortunately I have almost no experience in this field. Is here somebody who could help me analyze the spectrum? I would be very thankful
Hey everyone!
This is a forum for anyone who applied to the DoE CCI Summer 2021 Internships. Has anyone gotten a reply back from their labs? I am waiting on LLNL and SNL. I applied for Biotechnology, Engineering technology-Bio, and Energy Technology Bio.
-Feel free if you have heard back...
E. coli is one of the best studied and most widely used bacteria in biotechnology. This week, published in the journal Cell, researchers report having engineered the bacterium to be able to generate all of its carbon from CO2, opening the way toward using these bacteria for replacement of...
Over the past half decade, researchers have envisioned the https://www.physicsforums.com/insights/dont-fear-https://www.physicsforums.com/insights/dont-fear-crispr-new-gene-editing-technologies-wont-lead-designer-babies/-new-gene-editing-technologies-wont-lead-designer-babies/system as a way to...
I was reading RAPD kit manual by himedia and couldn't understand why the two primers should be at close proximity and in opposite orientation to actually amplify DNA.
'In RAPD analysis, for PCR to occur: a) the primers must anneal in a particular orientation (such that they point towards each...
Mosquitoes are responsible for spreading a number of human diseases (including malaria, Dengue fever, and Zika virus), and account for nearly half a million deaths per year worldwide. For many years, scientists have been investigating technologies that could help control or even eliminate...
'The MCSs of many vectors such as the pUC series are flanked by sequences complementary to a universal series of primers, the M13 forward and reverse primers. These priming sites are oriented such that extension of the primers annealed to these sites allows sequencing of both ends of an insert...
I am completely stumped by this question. I can't understand what is meant by complementation.
Here's an excerpt from Primrose:
'The activity of the enzyme b-galactosidase is easily monitored by including in the growth medium the chromogenic substrate 5-bromo- 4-chloro-3-indolyl-b-D-galactoside...
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