- #1
TytoAlba95
- 132
- 19
- Homework Statement
- During transgenesis, the location of genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of genes and their tissue specific transcription. The following are the possible methods used for the determination:
a. PCR
b. Southern blotting
c. Reverse transcriptase PCR
d.Western blotting
- Relevant Equations
- Ans: Southern blotting and RT-PCR
I have problem understanding these techniques with reference to the question:
a. PCR
b. Southern blotting
c. Reverse transcriptase PCR
d.Western blotting
My attempt:
d. Western blotting is not the right method here because it doesn't detect DNA or RNA transcript.
b. Southern blotting can be used to determine the copy number of the insert in the animal genome.
a. PCR can detect the presence of the gene but cannot be used to detect the copy number.
I don't understand how RT-PCR detects the tissue specific transcription level.
a. PCR
b. Southern blotting
c. Reverse transcriptase PCR
d.Western blotting
My attempt:
d. Western blotting is not the right method here because it doesn't detect DNA or RNA transcript.
b. Southern blotting can be used to determine the copy number of the insert in the animal genome.
a. PCR can detect the presence of the gene but cannot be used to detect the copy number.
I don't understand how RT-PCR detects the tissue specific transcription level.