I know its covered in most bio textbooks.But i can't udestand what they r saying ! I wanted like a simple explanation for dummies...Ygggdrasil said:This topic is covered in most molecular biology textbooks. For example, see https://www.ncbi.nlm.nih.gov/books/NBK26829/#_A1062_
Ygggdrasil said:
The purpose of charging tRNA is to prepare it for protein synthesis by attaching a specific amino acid to its 3' end through a process called aminoacylation. This amino acid will then be used to build a polypeptide chain during translation.
TRNA is charged with an amino acid through the action of specific enzymes called aminoacyl-tRNA synthetases. These enzymes recognize the specific tRNA and amino acid pair and catalyze the attachment of the amino acid to the tRNA's 3' end.
The CCA sequence is a highly conserved sequence found at the 3' end of all tRNA molecules. It stands for cytosine-cytosine-adenine and is important for the attachment of the amino acid during charging of tRNA.
The presence of the CCA sequence at the 3' end of tRNA allows for the aminoacyl-tRNA synthetase enzyme to easily recognize and bind to the tRNA molecule. This ensures that the correct amino acid is attached to the tRNA, as each amino acid has its own specific tRNA and aminoacyl-tRNA synthetase.
Yes, tRNA can be charged with multiple amino acids. Some tRNA molecules have a "wobble" base in their anticodon that allows them to bind to multiple codons and carry different amino acids. This allows for flexibility and efficiency in protein synthesis.