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vande060
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Homework Statement
Prepare 500ml of a reaction buffer containing .82% MgCl2, 62.5 mM glucose, 950 ATP, and 100mM Tris -Cl, pH 7.8 Use the stock solutions you have prepared
we had to prepare in previous problems:
500ml 1.75M glucose
25mL 2.5% glucose
2L of 1.8 Tris, pH 8.5
Homework Equations
C1V1 = C2V2
Pka Tris = 8.1
glucose mol mass = 180.16
ATP mol mass = 507.8
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The Attempt at a Solution
my calculation for MgCl2:
.82% = .82g/100ml = 8.2g/L
15% = 15g/100ml = 150g/L
C1V1=C2V2
150*x = 8.2*500
x = 28mL stck 15% MgCl2
My calculation for glucose
1.75M glucose = 315g/L
62.5mM = 11.2 g/L
C1V1=C2V2
11.2*500 = 315*x
x = 17.7 ml 1.75M glucose stock
Here is my calculation for ATP
5mM ATP = 3g/L
950nM ATP = .00048g/L
C1V1=C2V2
3*x = .00048*500
x =.08ml of stocK 5mM ATP
My calculation for the Tris I am not sure about, can I use my original pH 8.5 solution the prof asked me to prepare, or do I have to start over with the Tris acid and base components and use henderson H equation? does this all look right so far?