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Homework Statement
I just purified alkaline phosphatase, and my calculated molecular weight is 100000 g/mol, while literature value is 89000 g/mol. According to how others in my lab did calculations, my calculations are performed correctly, so I am left to explain why my purification is higher than expected.
Homework Equations
N/A
The Attempt at a Solution
I was thinking that since alkaline phosphatase can be inhibited by EDTA, my "purified" enzyme contains some EDTA, making the molecular weight higher.