- #1
saysua
- 2
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I think those who are well-immersed in the scientific community are familiar with the biological databases connected through the website www.ncbi.nlm.nih.gov.
I was just wondering if someone could possibly explain to me a little about the Blast database for me. IN a particular section of a homework assignment, Blast is needed to query for matching protein/gene sequences. The search for an unc. C elegan prtoein/gene sequence resulted in a bunch of data on species that have similar protein/gene sequences and alignments.
An example for the protein sequence results are:
Query 1 MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTA 60
MEHEKDPGWQYLRR+REQ+LEDQSKPYDSKKN WIPDPEEGYLAGEITATKGDQVTIVTA
Sbjct 1 MEHEKDPGWQYLRRSREQILEDQSKPYDSKKNCWIPDPEEGYLAGEITATKGDQVTIVTA 60
My questions are;
are each of the letters codons of amino acids?
In terms of its relationship to each other and to my initial protein query, what is query 1 and subject 1?
I'll deeply appreciate it if you could provide further insights into this database. (maybe there's a crashcourse or something on it somewhere?) My professor seems to be skimming ont he surface of the importance of these databases (blast/wormbase/flybase), but I want to know more about it since there is a good probability that I might find myself using it in the future.
Many thanks in advance!
I was just wondering if someone could possibly explain to me a little about the Blast database for me. IN a particular section of a homework assignment, Blast is needed to query for matching protein/gene sequences. The search for an unc. C elegan prtoein/gene sequence resulted in a bunch of data on species that have similar protein/gene sequences and alignments.
An example for the protein sequence results are:
Query 1 MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTA 60
MEHEKDPGWQYLRR+REQ+LEDQSKPYDSKKN WIPDPEEGYLAGEITATKGDQVTIVTA
Sbjct 1 MEHEKDPGWQYLRRSREQILEDQSKPYDSKKNCWIPDPEEGYLAGEITATKGDQVTIVTA 60
My questions are;
are each of the letters codons of amino acids?
In terms of its relationship to each other and to my initial protein query, what is query 1 and subject 1?
I'll deeply appreciate it if you could provide further insights into this database. (maybe there's a crashcourse or something on it somewhere?) My professor seems to be skimming ont he surface of the importance of these databases (blast/wormbase/flybase), but I want to know more about it since there is a good probability that I might find myself using it in the future.
Many thanks in advance!