How Do I Calculate Se Concentration from Multiple Calibration Curves?

[Beer-monster]

URGENT- Calibration curve question

Okay, so I'm a physicist doing a chem class so I'm a little out of my depth.

I've got a lab report to hand in but I'm a little stuck on the last part, working out the concentration of Se from a calibration curve. My problem is that the calibration curve is against the result from MS analysis, and I have three curves on the graph from 77, 78 and 82 Se, however the x-axis (based on my standards) is the concentration of Se not of the individual isotopes.

How do I work out the concentration of Se in my digest as a whole from the three curves, should I just work out the result from each curve and take and average?

Thanks

 

[GCT]

How do I work out the concentration of Se in my digest as a whole from the three curves, should I just work out the result from each curve and take and average?

I'm not absolutely sure what you're trying to do here. From the mass spectral analysis you should be able to acertain the abundances of the isotopes, your description of the calibration curve is unclear.

 

[Blueshift5]

for reaching out for help with your calibration curve question. It sounds like you have a good understanding of the concept of calibration curves, but are unsure of how to apply it in this specific situation. Let me try to break it down for you.

First, it's important to understand that a calibration curve is a graph that shows the relationship between the concentration of a substance and the measured response. In your case, the measured response is the result from MS analysis, and the substance is Se.

Now, since you have three curves on the graph for different isotopes of Se, it means that your MS analysis is able to detect all three isotopes. This is important because each isotope may have a different response to the concentration of Se. Therefore, it would not be accurate to simply take an average of the results from each curve.

Instead, you will need to use the equation of the calibration curve to calculate the concentration of Se in your digest. This equation will take into account the different responses from each isotope and give you a more accurate result.

If you are unsure of how to use the equation, I suggest reaching out to your instructor or a TA for further guidance. It's always better to ask for help and ensure accuracy in your results.

I hope this helps and good luck with your lab report!