- #1
tr111
- 3
- 0
Hi,
When we use an optical microscope, how do we roughly calculate the illuminated spot size on the sample ? Also what is the light power on that spot?
And what if I change to fluorescence microscope(epi illumination)? Cause the illuminating light go through the rear side of objective to the surface of specimen, also how do we calculate the spot size?
Thanks!
When we use an optical microscope, how do we roughly calculate the illuminated spot size on the sample ? Also what is the light power on that spot?
And what if I change to fluorescence microscope(epi illumination)? Cause the illuminating light go through the rear side of objective to the surface of specimen, also how do we calculate the spot size?
Thanks!