- #1
mtc1973
- 112
- 1
Okay so I have a complex setup that I hope I can convey.
I have 9 sites to which X can bind. 6 out of the 9 sites are active and 3 out of the 9 sites are inactive. I need 3 of the active sites to be bound to get the response I am looking for - which we will call EMAX.
So when I add a single X - the chance of it binding to an active site is 6/9 the chance of it binding to an inactive site is 3/9.
My probability knowledge is shaky - bear with me.
Assume that the binding is irreversible. So how many X do I need to add to be sure I have activated 3 active sites. Or more precisely, how many X do I need to add to get a >95% chance that 3 active sites are bound.
Then I want to go more complicated. Say I add 3 Y - which inactivates the sites. The chances are that 2 active sites will be inactivated and 1 inactive site will still be inactive with Y bound.
Now under these new conditions - how much X do I need to add to be sure 3 remaining active sites are occupied?
So I know it will be a probability - so I guess let's say that how much X do I need to add to have a greater than 95% chance that 3 active sites are now bound with X to get EMAX
I have 9 sites to which X can bind. 6 out of the 9 sites are active and 3 out of the 9 sites are inactive. I need 3 of the active sites to be bound to get the response I am looking for - which we will call EMAX.
So when I add a single X - the chance of it binding to an active site is 6/9 the chance of it binding to an inactive site is 3/9.
My probability knowledge is shaky - bear with me.
Assume that the binding is irreversible. So how many X do I need to add to be sure I have activated 3 active sites. Or more precisely, how many X do I need to add to get a >95% chance that 3 active sites are bound.
Then I want to go more complicated. Say I add 3 Y - which inactivates the sites. The chances are that 2 active sites will be inactivated and 1 inactive site will still be inactive with Y bound.
Now under these new conditions - how much X do I need to add to be sure 3 remaining active sites are occupied?
So I know it will be a probability - so I guess let's say that how much X do I need to add to have a greater than 95% chance that 3 active sites are now bound with X to get EMAX