- #1
BBoy.Goon
- 2
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I'm doing a science project about the antioxidant activity of red wines from my country. This days, I was going through the theoretical part ( the basics of spectrophotometry, photometric analysis ect. ) and I'm currently at the Beer-Lambert Law. I'm would like to know the whole mathematical path that is used so that starting from this two equations: A = 2 - log10 %T and A = [tex]\epsilon[/tex]bc,
where:
A is absorbance, ( no units )
T is transmittance, ( no units )
[tex]\epsilon[/tex] is molar absorbtivity, ( L mol-1cm-1 )
b is the length of the sample ( path length of the cuvette ) ( cm ) and
c is the concentration of the compound in solution ( mol L-1 )
the equation %T = e -[tex]\epsilon[/tex]bc is deduced.
note: the molar absorbtivity is represented by the letter epsilon from the Greek alphabet and it's not superscripted as it looks like.
Thanks in advance
where:
A is absorbance, ( no units )
T is transmittance, ( no units )
[tex]\epsilon[/tex] is molar absorbtivity, ( L mol-1cm-1 )
b is the length of the sample ( path length of the cuvette ) ( cm ) and
c is the concentration of the compound in solution ( mol L-1 )
the equation %T = e -[tex]\epsilon[/tex]bc is deduced.
note: the molar absorbtivity is represented by the letter epsilon from the Greek alphabet and it's not superscripted as it looks like.
Thanks in advance