- #1
Rafimah
- 14
- 1
Homework Statement
Hello,
I've been using a light spectrometer to try to measure the reflectance of a sample of a protein I have on a glass slide. I followed the manual's instructions and first took a dark spectrum and a reference spectrum. However, the intensity was too high (far above the maximum recorded value of 4095 counts), so I used an aperture to reduce the amount of light released by the lamp I was using. This worked well, as for the reference spectrum I needed a peak at 3500 counts. However, when I looked at thee percent transmission, I noticed it was incredibly high (around 1000%). I placed a sample in there, just to test if that would improve anything, but it did not. Does anyone have experience with this and measuring the reflectance of samples?
The Attempt at a Solution
I thought that the angle of the slide might be relevant. Does anyone know how important the angle is in light spectrometry? Unfortunately, the sample is placed in a holder and, while the slide can touch anything, the sample itself cannot be contaminated, so I was forced to place it at a roughly 45 degree angle.