Is this correct? A molar conversion for NAD+ at 50 micromolar concentration

Chemistry
Thread starter Peterz
Start date Oct 14, 2023

In summary, the document discusses the molar conversion of NAD+ at a concentration of 50 micromolar, exploring its implications for biochemical reactions and potential applications in research. It emphasizes the importance of accurate concentration measurements for effective utilization in experiments involving NAD+.

Oct 14, 2023

Peterz

Homework Statement: Convert 50 micromolar NAD+ to ng/ml

Relevant Equations: I mole NAD+ = 660 grams
1 micromolar = 1 micromole/L
1,000,000,000 nanograms/gm

660 grams/mole for NAD+
50 micromolar is 0.00005 mole/L
0.00005 mole/L x 660 gm/mole = 0.033 gm/L
1,000,000,000 ng/gm x 0.033 gm/L = 33,000,000 ng/L
33,000,000 ng/L x 1L/1000ml = 33,000 ng/ml Is this correct?

Last edited: Oct 14, 2023

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Oct 14, 2023

Borek

Mentor

looks OK to me

FAQ: Is this correct? A molar conversion for NAD+ at 50 micromolar concentration

What is a molar conversion?

A molar conversion refers to the process of converting a given concentration of a substance from one unit of measurement to another, typically involving moles. It often requires knowledge of the substance's molecular weight and the volume of the solution.

How do you calculate molar concentration?

To calculate molar concentration, you use the formula: Molarity (M) = moles of solute / liters of solution. For NAD+ at a 50 micromolar concentration, you would need to convert micromoles to moles (50 micromoles = 50 x 10^-6 moles) and then divide by the volume of the solution in liters.

What is the molecular weight of NAD+?

The molecular weight of NAD+ (Nicotinamide adenine dinucleotide) is approximately 663.43 g/mol. This value is crucial for converting between mass and moles in molar conversions.

How do you convert micromolar to molar?

To convert micromolar (µM) to molar (M), you divide by 1,000,000 since 1 M = 1,000,000 µM. Therefore, 50 µM is equivalent to 50 x 10^-6 M or 0.00005 M.

Is 50 micromolar a high concentration for NAD+ in biochemical assays?

No, 50 micromolar is generally considered a low to moderate concentration for NAD+ in biochemical assays. It is often used in enzymatic reactions and studies involving cellular metabolism.