Molisch & Fehling Tests: Comparing Results

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In summary, the Molisch and Fehling tests are used to detect the presence of carbohydrates in a solution. The Molisch test is non-specific and detects any type of carbohydrate, while the Fehling test is specific to reducing sugars. Both tests involve a chemical reaction that results in a color change. However, they are not specific to any particular type of carbohydrate and cannot be used for quantitative analysis. Factors such as the presence of other substances and low carbohydrate concentration can affect the results of these tests. To determine the exact concentration of carbohydrates, a separate quantitative method must be used.
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Hey, what could be a compund that gives a positive Molisch test but a negative fehling's test?
 
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Hello, I haven't remembered what is the Molisch test, but a negative Fehling's test indicates that the compound does not have any oxidizable group, especially the aldehyde one.
 
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There are a few possible compounds that could give a positive Molisch test but a negative Fehling's test. One example is a non-reducing sugar such as sucrose. The Molisch test detects the presence of any carbohydrate, including non-reducing sugars, while the Fehling's test specifically detects reducing sugars. Another possibility is a compound that contains a ketone group, which would give a positive Molisch test but would not react with the copper ions in Fehling's solution. Additionally, certain aromatic compounds can give a positive Molisch test due to the presence of phenolic groups, but would not react with Fehling's solution. Ultimately, the results of these tests depend on the functional groups present in the compound being tested, so it is important to consider the specific properties of the compound in question.
 

FAQ: Molisch & Fehling Tests: Comparing Results

What is the purpose of the Molisch and Fehling tests?

The Molisch and Fehling tests are both used to detect the presence of carbohydrates in a solution. The Molisch test specifically detects the presence of any type of carbohydrate, while the Fehling test is more specific and only detects the presence of reducing sugars.

How do the Molisch and Fehling tests differ?

The Molisch test involves adding a few drops of alpha-naphthol and concentrated sulfuric acid to a solution, which reacts with carbohydrates to form a purple ring. In the Fehling test, the solution is mixed with Fehling's reagent, which contains copper ions, and heated. If reducing sugars are present, they will reduce the copper ions, causing a color change from blue to red.

Can the Molisch and Fehling tests be used to identify specific types of carbohydrates?

The Molisch test is not specific to any particular type of carbohydrate, while the Fehling test is only specific to reducing sugars. Other tests, such as the Benedict's test, can be used to specifically identify glucose and other reducing sugars.

What factors can affect the results of the Molisch and Fehling tests?

The presence of other substances, such as proteins or acids, can interfere with the results of both tests. Additionally, if the concentration of the carbohydrate is too low, the tests may not produce a clear positive result. It is important to follow the instructions carefully and perform control tests to ensure accurate results.

Are the Molisch and Fehling tests quantitative?

No, both tests are qualitative and only indicate the presence or absence of carbohydrates in a solution. They cannot be used to determine the exact concentration of carbohydrates present. To quantify the amount of carbohydrates, a separate method, such as titration or spectrophotometry, must be used.

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