- #1
spacetime
- 119
- 2
Hey, what could be a compund that gives a positive Molisch test but a negative fehling's test?
The Molisch and Fehling tests are both used to detect the presence of carbohydrates in a solution. The Molisch test specifically detects the presence of any type of carbohydrate, while the Fehling test is more specific and only detects the presence of reducing sugars.
The Molisch test involves adding a few drops of alpha-naphthol and concentrated sulfuric acid to a solution, which reacts with carbohydrates to form a purple ring. In the Fehling test, the solution is mixed with Fehling's reagent, which contains copper ions, and heated. If reducing sugars are present, they will reduce the copper ions, causing a color change from blue to red.
The Molisch test is not specific to any particular type of carbohydrate, while the Fehling test is only specific to reducing sugars. Other tests, such as the Benedict's test, can be used to specifically identify glucose and other reducing sugars.
The presence of other substances, such as proteins or acids, can interfere with the results of both tests. Additionally, if the concentration of the carbohydrate is too low, the tests may not produce a clear positive result. It is important to follow the instructions carefully and perform control tests to ensure accurate results.
No, both tests are qualitative and only indicate the presence or absence of carbohydrates in a solution. They cannot be used to determine the exact concentration of carbohydrates present. To quantify the amount of carbohydrates, a separate method, such as titration or spectrophotometry, must be used.