Optical Tweezer Laser Focus/Alignment Troubleshooting

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wheatie
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TL;DR Summary
Optical Tweezer Trapping Help
Currently we have an optical tweezer setup that has a beam expander + dichroic mirror that fills the aperture of the objective, and a microscope that is able to look into the aperture and see a diameter of about 30 microns.
We have been stuck for a couple weeks now, as we do not know if our laser is actually trapping any particle/if the trapping is occurring within the fov of the camera.
Our questions would be:
How can we tell if our laser is focused.
How can we tell if our camera and laser is focused at the same point.

I've included a photo of our current setup.
Thank you!

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