gen.hope55 said:I am a university student and have this question for you guys.
Why do dead cells under phase contrast microscope do not show a bright halo?
Thanks for your help
Gen.hope55
Phase contrast microscopy is an imaging technique used to enhance contrast in transparent specimens. It works by using a specialized condenser and objective to transform slight differences in refractive index into differences in brightness, making it possible to view structures that would otherwise be invisible under traditional brightfield microscopy.
The main advantage of phase contrast microscopy is its ability to visualize transparent specimens without the need for staining or other sample preparation techniques, which can alter or damage the specimen. It also allows for live cell imaging and provides high contrast images with minimal background noise.
Phase contrast microscopy is commonly used to view biological specimens, such as cells, tissues, and microorganisms. It can also be used to study non-biological samples, such as thin films, colloidal suspensions, and polymers.
One limitation of phase contrast microscopy is its inability to provide depth information, as all structures in the focal plane appear with the same brightness. It also has lower resolution compared to other microscopy techniques, such as fluorescence microscopy.
Phase contrast microscopy differs from other techniques in that it does not require staining or labeling of the specimen, making it a non-destructive imaging technique. It also provides high contrast images of transparent specimens, which may not be visible under brightfield or other traditional microscopy methods.