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sotellme
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Where should I prepare the media? In the laminar flow hood or on an opened table (risks for contamination?) ?
Media preparation for mammalian cells involves creating a nutrient-rich solution that provides the necessary nutrients and growth factors for the cells to grow and divide.
The components of media for mammalian cells typically include a base medium, such as DMEM or RPMI, essential amino acids, vitamins, glucose, and serum for added growth factors.
The appropriate media for mammalian cells depends on the type of cells and their specific growth requirements. Consult the literature or manufacturer's instructions for recommended media formulations.
The process for preparing media for mammalian cells involves sterilizing all equipment and materials, accurately measuring and mixing the components, and adjusting the pH and osmolality to match the cells' requirements.
Prepared media for mammalian cells should be stored in a sterile container and kept at 4°C if using within a few days. For longer storage, it should be frozen at -20°C or -80°C. Prior to use, the media should be thawed and warmed to room temperature.