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samzorz
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Homework Statement
We have a 100 mL of 10 mM Tris-HCl, 1 mM EDTA, pH = 7.4
We're supposed to add PMSF to a final concentration of 1 mM and 2-mercaptoethanol to a final concentration of 1 mM. The stock solution of PMSF is .10 M PMSF in absolute ethanol. The 2-mercaptoethanol is also in the hood, it is 14.3 M.
We're trying to find the volume of .10 M PMSF needed and the volume of 2-mercaptoethanol needed
Homework Equations
M1V1=M2V2?
The Attempt at a Solution
Not sure how we can use the equation if we have 2 concentrations