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Does anyone know the molecular detail of how it works?
http://micro.nwfsc.noaa.gov/protocols/rbcl.html
It is a method to make E. coli cells competent so that they are willing to take up DNA from their environment. So I guess the RbCl disrupts the plasma membrane in a certain way.. but why the other specific salts? The glycerol must be make the cells freezable.. MOPS is a detergent..
I don't know exactly :P
http://micro.nwfsc.noaa.gov/protocols/rbcl.html
It is a method to make E. coli cells competent so that they are willing to take up DNA from their environment. So I guess the RbCl disrupts the plasma membrane in a certain way.. but why the other specific salts? The glycerol must be make the cells freezable.. MOPS is a detergent..
I don't know exactly :P
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