- #1
kalladin
- 74
- 0
Hi, I don't know if anyone here can help me. But I've lately been silver staining chromosome spreads for EM on plastic coated slides. However, when I do the water rinses after the staining procedure, my plastic coating just comes off and it infuriates me to no end as the whole spreading/staining procedure takes me 8 hours (6 on a good day). Does anyone have any experience and/or advice for me? I've been staining them with silver nitrate (50%) and a nylon mesh cover for 1.5 hours and adding additional stain every 15 minutes. I don't 'think this has anything to do with the temperature. I DO see some weird stuff happening that doesn't happen with normal glass slides- like how the solution bubbles up (i'm thinking some of the stain went under the plastic)?
I will appreciate any help!
Kevin
I will appreciate any help!
Kevin