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caa4444
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I'm working on an experiment to measure the quenching of an oxygen-sensitive dye. The excitation light is blue and the light fluoresced is red. When I collect data, there is always a peak for blue and red, which makes it impossible to measure really the quenching in low dye concentrations because the blue overlaps the small red emission. I'm thinking of using a polarizing film, but don't know the best setup.
Below is the current setup:
[film w/ dye] — |glass wall| — spectrometer & blue light source
Can you guys tell me how to minimize the pickup of blue light without affecting red light pickup?
Below is the current setup:
[film w/ dye] — |glass wall| — spectrometer & blue light source
Can you guys tell me how to minimize the pickup of blue light without affecting red light pickup?