- #1
physicisttobe
- 56
- 13
- Homework Statement
- Questions about Ran GTPase
- Relevant Equations
- ...
Hi everyone!
There is a question that I can't solve.
Ran GTPase controls nuclear import of proteins and is present at a much higher concentration than its GAP (GTPase activating protein) and GEF (guanine nucleotide exchange factor) proteins. Imagine a mutation in the Ran GTPase resulting in an extremely tight binding between GTPase and its GEF and a very slow dissociation. What happens as a result?
The answer to this question is: GDP, because the tightly bound GEF's will be unavailable to act on further Ran molecules.
But why GTP? Isn't it GDP? I mean, there is a binding between GDP and GEF, and this binding forms GTP+GEF so that GTP can bind to another cargo protein (NLS). I do not understand this. Could you explain me that?
There is a question that I can't solve.
Ran GTPase controls nuclear import of proteins and is present at a much higher concentration than its GAP (GTPase activating protein) and GEF (guanine nucleotide exchange factor) proteins. Imagine a mutation in the Ran GTPase resulting in an extremely tight binding between GTPase and its GEF and a very slow dissociation. What happens as a result?
The answer to this question is: GDP, because the tightly bound GEF's will be unavailable to act on further Ran molecules.
But why GTP? Isn't it GDP? I mean, there is a binding between GDP and GEF, and this binding forms GTP+GEF so that GTP can bind to another cargo protein (NLS). I do not understand this. Could you explain me that?