- #1
Alice Jin
- 18
- 0
Dear everyone,
I'm wondering what the diffraction limited spot is.
When a laser(CW) is used to excite a fluorescent single molecule, the fluorescence from a single molecule is shown in the CCD camera. The size of fluorescence molecule is determined by Airy Disc which comes from diffraction limited spot. Is it right?
Does anyone explain this? Please~~ Thank you.
I'm wondering what the diffraction limited spot is.
When a laser(CW) is used to excite a fluorescent single molecule, the fluorescence from a single molecule is shown in the CCD camera. The size of fluorescence molecule is determined by Airy Disc which comes from diffraction limited spot. Is it right?
Does anyone explain this? Please~~ Thank you.