Why Does Fluorescence Decrease During the Lag Phase in 3T3 Cell Growth Curves?

In summary, the speaker has observed a decrease in fluorescence values during the lag phase of a cell growth curve, which is not something they have encountered before. They have considered the possibility of cell death occurring at a higher rate than replication, but this does not seem to explain the decrease. The speaker is seeking advice and resources to better understand this phenomenon. They have also been asked if they have conducted statistical analysis and if the decrease is statistically significant.
  • #1
Redmac
1
0
Hi All,

Having plotted a cell growth curve (fluorescence vs days), I have found that during the lag phase, the fluorescence values decrease. I haven't come across this previously during lectures and can't seem to find any examples online to help explain it. My only thought was that cell death occurred at a higher rate than replication whilst the cells were attaching to the culture flask but the decrease occurs between 1 and 3 days so this doesn't seem like a possible explanation. As a side note, 3T3 cells were being used during the experiment.

I'd appreciate any advice on this or resources that may be useful.

Many Thanks
 
  • #3
Have you run statistical analysis on the fluorescence values? Is it statistically significant decrease? Can you provide the raw data? It could just be normal variance or noise that you're interpreting as a decrease.
 

Related to Why Does Fluorescence Decrease During the Lag Phase in 3T3 Cell Growth Curves?

1. What is a cell growth curve analysis?

A cell growth curve analysis is a scientific method used to measure and track the growth of cells over time. This can provide valuable information about the growth rate, division rate, and other characteristics of cells.

2. How is a cell growth curve analysis performed?

A cell growth curve analysis involves taking regular measurements of cell growth over a period of time. The cells are typically grown in a controlled environment, such as a petri dish, and their growth is monitored through techniques such as microscopy or cell counting.

3. What are the applications of cell growth curve analysis?

Cell growth curve analysis has many applications in various fields of research, such as cell biology, microbiology, and pharmacology. It can be used to study the effects of different substances on cell growth, determine the optimal conditions for cell growth, and track the growth of cancer cells.

4. What information can be obtained from a cell growth curve analysis?

A cell growth curve analysis can provide information about the growth rate, doubling time, and saturation point of cells. It can also reveal any changes in growth patterns due to external factors, such as the presence of a drug or changes in the environment.

5. How is data from a cell growth curve analysis analyzed?

Data from a cell growth curve analysis is typically plotted on a graph with time on the x-axis and cell growth on the y-axis. This allows for the visualization of growth patterns and the determination of important parameters, such as growth rate and doubling time. Statistical analysis can also be performed on the data to identify any significant differences between experimental groups.

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