Microscope Definition and 281 Threads

Homework Statement 2. Homework Equations F-number is f/d x0 = 1.2λ(fnumber) The Attempt at a Solution [/B] Fnumber1 = 0.2 Fnumber2 = 0.6 x01 = 180nm x02 = 540nmThese values seem large for depth resolution, is this correct?

Homework Statement A compound microscope has an objective of focal length 12 mm and an eyepiece of focal length 50 mm. The lenses have a separation of 90 mm and an object of height 0.3 mm is placed 15 mm from the objective. Calculate: (a) the distance of the intermediate image from the...

I have very strong astigmatism, for a 17-year-old. I wear toric contacts, which is supposed to help people with astigmatism. I take a college biology class and today we were looking into light microscopes. I have always struggled with looking in microscopes, but today felt so much worse. After...

Homework Statement They were going to purchase an electron microscope, and wanted the electrons velocity to be as high as 25% of the speed of light after acceleration. There was a microscope at hand that had an accelerating voltage of 40 kV. a) How large is the electrons relativistic mass when...

https://www.dropbox.com/sc/eo2c8j4kd12pdvn/AACGJ8YZvk35BSX1BsBoKIIsa Please view the above image. I am getting awesome contrast on the periphery of the image but hardly any in the center. The sample is an H and E stained tissue and I just got the microscope in today. I am a rookie with...

when physicists say that they apply voltage to the tip or the sample, do they mean that the tip or sample become electrically charged?

Looking at Ben Krasnow's youtube video on breaking down the parts and general cost for a scanning electron microscope, he lists a raster scan generator, is that able to produce the highest resolution images still? Or is there another more modern technology for this purpose? Otherwise, how has...

Why is the focal length of the objective lense is less than that of the eye piece in a compound microscope?

thankyou to anyone reading this. I am starting honours in physics and have been given the opportunity to do a project that leads to a phd in the condensed matter theory. The guy I will be working with seems to specifically do work around the theory involved in electron microscopes. hes a great...

I am using a Renishaw inVia confocal microscope to study emission spectrum of molecules. The excitation wavelength of laser that I use is 532nm. However, I met with a very basic problem. When I use the same material (say plain silicon), and use different objectives (20X, 50X, 100X), then I will...

Homework Statement Basically, derive the formula ## m = \frac{ 25 cm}{f_e} \frac{L}{f_o} ## using ray matrices. This just has variable tube length and assumes eye to object distance is 25 cm. Homework Equations Ray matrices: ## \left[ \begin{array}{cc} 1 & d \\ 0 & 1 \end{array} \right] ##...

Can the Heisenberg's Gamma Ray Microscope thought experiment derive the Uncertainty Principle precisely? Most derivations I find, the uncertainty is roughly 2h, whereas the uncertainty principle is "hbar over 2". Is there anywhere where there is more precise calculations to obtain "hbar over 2"...

Can someone tell me the specs of the pathology microscope lenses both objective and eyepiece/ocular lenses . Objective lenses magnification are as follows; 10X 45X 100X Eyepiece lenses magnification are as follows; 15X 10X Specifications should mention details about their Focal length Radius of...

I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. And my goal is image this by electron tomography by collecting a dual tilt series of 120 images. Other experimental...

I have recorded a micrograph of a 2-D array at a magnification of 43,000x on my DE-20 digital camera, which has a 6.4 μm pixel size and a frame size of 5120 × 3840 pixels. This magnification is correct at the position of the camera. I then compute the Fourier transform of the image. What is the...

Homework Statement The barrel of a compound microscope is 15.9 cm in length. The specimen will be mounted 1.17 cm from the objective, and the eyepiece has a 5.00 cm focal length. Determine the focal length of the objective lens.Homework Equations I am using the mirror equation. The Attempt at...

Homework Statement First, thanks in advace. Let us consider a microscope where the objective L1 has f1=20mm and magnification 10x. In the image plane is located a diafragm M with diameter 19mm (see fig). The size of the CCD is 4,8mm (vertical) x 5,6mm (horizontal). 20mm before of the CCD...

Is there any usage for a measuring microscope in an electricity laboratory as a part of any experiment? We have a Mitutoyo measuring microscope in our fundamental electricity physics lab which is a course for sophomores. We used it for apparent depth in our optics lab which is retired now! I'm...

is magnification negative for a compound microscope at infinity

Not sure if this is the right place for this thread but, have you guys ever made anything cool at home? I was planning on making an inverted microscope out of a webcam by just taking the lens out and flipping it over and setting it up like a microscope.

I noticed that the ray diagram for the "how the compound microscope works" and "how the telescope works" is wrong in my government sanctioned physics textbook(and countless other books and websites!) The diagram printed was this- Name: Physics Standard XI Printed by: Maharastra State Board of...

Homework Statement Your molecular biology lab studies proteins, and you're frustrated because your microscopes can't quite resolve crystallized proteins. A sales rep touts the advantages of an expensive microscope using 200-nm ultraviolet light, saying you'll be able to resolve structures less...

I can only find micrographs (photographs of microstructure under electron microscope) of non-transparent glasses. Is it impossible top be able to see the internal structure of a transparent material? If anyone can tell me what I'm doing wrong or find me one that would be great! I'm looking...

The question is: A microscope has a 12.0 X eyepiece and a 59.0X objective lens 20.0cm apart. Calculate (a) the total magnification, (b) the focal length of each lens, and (c) where the object must be, for a normal relaxed eye to see it in focus. I have solved (a) easily through M = moMe...

Homework Statement The image formed by a microscope objective with a focal length of 5 mm is 160mm from its second focal point. The eyepiece has a focal length of 26mm. a.what is the angular magnification of the microscope?Homework Equations Mtotal=M_e*m_o m_o= linear magnification of...

Homework Statement The problem along with its solution is attached as ProblemSolution.jpg. Homework Equations Δx = λ/sinθ (Eq. 1) Δp_x = (h/λ)(sinε) (Eq. 2) The Attempt at a Solution In Wikipedia, I found this article: http://en.wikipedia.org/wiki/Heisenberg's_microscope which uses...

Homework Statement The problem along with its solution are included as ProblemSolution.jpg. Homework Equations (Eq. 1) Δx ≈ λ/(sinθ) (Eq. 2) Δp_x ≈ 2h(v/c)(sinθ) = 2(h/λ)(sinθ) (Eq. 3) Δx Δp = ħ/2 The Attempt at a Solution I am confused about several things. Firstly, geometrically...

Hi there As is known using non-infinity corrected objectives in infinity corrected microscopes is not a good idea because of many reasons (image quality will be degraded, back focal plane will be in wrong place, parfocal distance is not preserved, etc.). What about building infinity corrected...

I've been going through some past papers in preparation for my exam tomorrow. We have not been given the past paper answers so i was wondering if anyone could help me with some of the answers. I will post multiple questions here but help with any of them would be greatly apprechiated Homework...

Homework Statement You are building a compound microscope with an objective lens of focal length 0.70 cm and an eyepiece lens of focal length 5.0 cm. You mount the lenses 18 cm apart. What is the maximum magnification of your microscope? Homework Equations...

What does the piezoelectric scanner do? Does it record the electric current tunnelled by the tip or is it just used to move the tip in response to electric currents?

Homework Statement A microscope has a 1.6 cm focal-length eyepiece and 0.81 cm objective. Assume a relaxed normal eye. (a) Calculate the position of the object if the distance between the lenses is 16.0 cm. _____ cm (from the objective) (b) Calculate the total magnification. ________...

Dear All I am trying to measure the spectrum of an incandesant 6V 15W tungsten microscope lamp. I have a reasonably old scanning monochromator (diffraction grating based) coupled to a fibre optic cable and it has given me the spectrum...

Is it possible to see twins in Si under an optical microscope?

I'm not asking for what reflection and refraction are or the usual law governing it, but I would like to understand what they represent at the quantum atomic, molecular level? In a mirror is it about photons absorbed and emitted with the same wavelength and same direction through atomic electron...

Homework Statement A microscope has a 13.0 x eyepiece and a 57.0 x objective lens 20.0 cm apart. Calculate the focal length of each lens. Where the object must be for a normal relaxed eye to see it in focus? Homework Equations M=m(objective)*m(eyepiece)=f(objective)/f(eyepiece)...

what is the depth of field of a microscope and how is it different from the depth of focus of the microscope.

Listening to lecture today in my E&M class, the professor was giving us the derivation of the micro description of current. I=neAv_d Somewhere along the line he comes to v_d=at that "t" might be a tau, I can't decipher my scribble. He said he isn't really sure how they get that particular...

Why in telescopes and microscopes the ocular distance is such that it creates virtual images instead of real images?

1. Homework Statement A Homemade compound microscope has, as objective and eyepiece, thin lenses of focal lengths 1cm and 3cm, respectively. An object is situated at a distance of 1.20cm from the objective. If the virtual image produced by the eyepiece is 25cm from the eye, compute (a.) the...

Hi Guys, Ive been doing some work with E. Coli recently and I would like to image them under a scanning electron microscope. There are lots of papers with lots of different methods to prepare them for such a feat... but has anyone here actually done it and gotten a decent image? If so what...

Hi, I am looking for some detailed electron microscop images of HIV virus itself. I need it bigger and more detailed then this: http://www.allvoices.com/contributed-news/9114517/image/80168820-hiv-virus-electron-micro-graph I would like to have some picture, not computer render or...

Looking for a rough distance scale over which statistical methods begin to work, and macroscopic thermodynamic variables begin to make sense. Thanks

My text explains that free float frames are locally indistinguishable from flat space-time in the sense that in a small enough region measuring instruments will be unable to detect gravitational tidal forces. This region may be tiny if tidal forces are large or if measuring instruments are...

I have been using a relatively cheap microscope (My first Lab Microscope) to look at a sample of my semen. Under 40x magnification I see a lot of motionless sperm and generally not enough sperm floating about compared to what I see from other microscope videos of sperm floating (no pun) on the...

Homework Statement To examine a particular blood sample in biology lab, a student uses a compound microscope set to have a magnification of -4530. The objective and eyepiece are both attached to a tube which is 18 cm in length and the eyepiece has a focal length of 2.62 cm. The near point of...

I know an electron microscope can collect x-rays emitted by electron holes, backscattered electrons as well as secondary electrons. I get that an electron's wavelength is much smaller than a photons, thus you get finer image resolution, but how exactly does a shorter wavelength mean a finer...

I just link my microscope (Olympus CX31) with CCD (Sony), the staff 's company installed "Ulead program" in computer linked with CCD to capture the picture from microcope. The problem is when the picture shown in the computer, it is already too enlarged that we can't see the wider view as we...

Homework Statement A biologist is using a high power optical microscope wishes to improve the resolution by staining the the specimen being viewed. which color stain would allow the biologist to achieve the better resolution: red or blue? Homework Equations The Attempt at a...

Hello, I am looking for some expert advice from the community. I am preparating a planar SERS surface using polycrystalline gold as a substrate. It will be roughened with oxidation-reduction cycles. I want to measure the sers substrate enhancement factor (SSEF) using pyridine as a reporter. I...