How Does a Mass Spectrometer Calculate the Path of Protein Fragments?

Your Name]In summary, the problem asks for the distance between two points in a mass spectrometer where a protein fragment enters and exits a magnetic field at a given speed and with a given mass. To solve this, the equation m=(qBr)/V is used, but the values for mass and velocity may need to be converted into proper units. Once the radius is calculated, it can be multiplied by 2 to find the distance between the two points.
  • #1
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Homework Statement


A mass spectrometer is designed to separate protein fragments. The fragments are ionized by removing a single electron and then enter a 0.80 T uniform magnetic field at a speed of 2.3*10^5 m/s
If a fragment has a mass that is 85 times the mass of the proton, what will be the distance between the points where the ion enters and exits the magnetic field?

Homework Equations





The Attempt at a Solution

I used m=(qBr)/V also tried r= (2mv/2qB^2)^.5

and then multipid r *2 to get d? Nothing is working. The value m I'm using is 1.4*10^-25, B=.8, q=1.6*10^-19, v=2.3*10^-5?

Help.
 
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  • #2


Dear forum post author,

Thank you for your question. It seems like you are on the right track with using the equation m=(qBr)/V to solve this problem. However, there may be a few errors in the values you are using.

Firstly, the mass of the protein fragment should be given in kilograms, not in arbitrary units like 1.4*10^-25. So you may need to convert the given mass of 85 times the mass of the proton into kilograms.

Secondly, the velocity should also be given in meters per second, not in an arbitrary unit like 2.3*10^-5. So you may need to convert the given speed of 2.3*10^5 m/s into meters per second.

Once you have converted the values to the correct units, you can plug them into the equation m=(qBr)/V and solve for the radius r. Then, as you mentioned, you can multiply the radius by 2 to get the distance between the points where the ion enters and exits the magnetic field.

I hope this helps. If you are still having trouble, please provide your updated calculations and we can work through them together.

 
  • #3


I understand the importance of accurately solving problems and finding the correct solution. Based on the information provided, I can suggest the following steps to solve this problem:

1. First, let's clarify the given values:
- Mass of the fragment = 85 times the mass of the proton = 85 * 1.67 * 10^-27 kg ≈ 1.42 * 10^-25 kg
- Magnetic field strength = 0.80 T
- Velocity of the fragment = 2.3 * 10^5 m/s

2. Now, let's use the equation m = (qBr)/V to calculate the charge (q) of the fragment. We know the mass (m), magnetic field strength (B) and velocity (V), so we can rearrange the equation to solve for q.
q = mV/(Br) = (1.42 * 10^-25 kg * 2.3 * 10^5 m/s)/(0.80 T * r)

3. We also know that the charge of the fragment (q) is equal to the charge of an electron (e) multiplied by the number of electrons removed (n). In this case, only one electron is removed, so n = 1. Therefore, we can write:
q = ne = (1)(1.6 * 10^-19 C) = 1.6 * 10^-19 C

4. Now, we can substitute this value of q in the previous equation and solve for r:
r = (mV)/(Be) = (1.42 * 10^-25 kg * 2.3 * 10^5 m/s)/(0.80 T * 1.6 * 10^-19 C) ≈ 0.11 m

5. Since we want the distance between the points where the ion enters and exits the magnetic field, we can multiply r by 2 to get the total distance:
d = 2r ≈ 0.22 m

Therefore, the distance between the points where the ion enters and exits the magnetic field is approximately 0.22 meters. I hope this helps!
 

FAQ: How Does a Mass Spectrometer Calculate the Path of Protein Fragments?

1. What is a mass spectrometer?

A mass spectrometer is a scientific instrument used to measure the mass and relative abundance of atoms and molecules in a given sample. It works by ionizing the sample and separating the ions based on their mass-to-charge ratio.

2. How does a mass spectrometer work?

A mass spectrometer works by first vaporizing and ionizing a sample using an ion source. The ions are then accelerated through an electric or magnetic field and separated based on their mass-to-charge ratio. Finally, the separated ions are detected and their abundance is measured.

3. What are the applications of a mass spectrometer?

Mass spectrometers have a wide range of applications in various fields such as chemistry, biology, medicine, and environmental science. Some common uses include identifying unknown compounds, determining the molecular structure of a substance, and measuring isotopic ratios.

4. What are the different types of mass spectrometers?

There are several types of mass spectrometers, including time-of-flight, quadrupole, magnetic sector, and ion trap. Each type has its own advantages and applications, but they all work based on the same principles of ionization, separation, and detection.

5. How accurate are mass spectrometers?

The accuracy of a mass spectrometer depends on various factors, including the type of instrument, the quality of the sample, and the skill of the operator. However, modern mass spectrometers can provide extremely precise and accurate measurements, with errors as low as 0.01%.

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