Which Negative Stain is Best for Sensitive Large Molecular Complexes in EM?

AI Thread Summary
The discussion centers on preparing a sensitive large molecular complex for electron tomography imaging, specifically using low ionic strength solutions due to the complex's integrity being affected by ionic strength. The user plans to collect a dual tilt series of 120 images but faces the challenge of lacking a cold specimen holder, necessitating room temperature experimentation. The main inquiry is about selecting the appropriate negative stain for specimen preparation, with a focus on the implications of the choice. The conversation also touches on the nature of the question, with some participants questioning whether it is a homework assignment and suggesting that effort in formulating the question could lead to better assistance.
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I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. And my goal is image this by electron tomography by collecting a dual tilt series of 120 images. Other experimental constraints are that I lack a cold specimen holder and thus must to this experiment at room temperature. From the possible negative stains that are available generally, which should I choose to prepare my specimen and why?
 
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You forgot to change the last "your" to "my". Is this a homework question?
 
Pythagorean said:
You forgot to change the last "your" to "my". Is this a homework question?
Yes. But can you help me somehow?
 
No, but someone who would be able to help you would probably appreciate some effort in answering the question on your part.
 

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