In transfer functions, substituting s with jw transforms G(s) into G(jw) to analyze the system's behavior in the frequency domain. This substitution links the Laplace transform to the Fourier transform, allowing for the extraction of gain and phase information at specific frequencies. The gain of a linear system at frequency ω can be determined by applying a sinusoidal input and observing the output amplitude. A linear system adheres to the principle that the sum of inputs equals the sum of outputs, while a time-invariant system maintains consistent output delays regardless of input shifts. Understanding these concepts is crucial for analyzing system dynamics effectively.